Dynamics and Hydration Explain Failed Functional Transformation in Dehalogenase Design

Authors

Sýkora, J., Brezovský, J., Koudeláková, T., Lahoda, M., Fořtová, A., Chernovets, T., Chaloupková, R., Štěpánková, Prokop, Z., Kutá Smatanová, I., Hof, M., Damborský, J.

Source

Nature Chemical Biology 10

Abstract

We emphasize the importance of dynamics and hydration for enzymatic catalysis and protein design by transplanting the active site from a haloalkane dehalogenase with high enantioselectivity to nonselective dehalogenase. Protein crystallography confirms that the active site geometry of the redesigned dehalogenase matches that of the target, but its enantioselectivity remains low. Time-dependent fluorescence shifts and computer simulations revealed that dynamics and hydration at the tunnel mouth differ substantially between the redesigned and target dehalogenase.

Source

Sýkora, J., Brezovský, J., Koudeláková, T., Lahoda, M., Fořtová, A., Chernovets, T., Chaloupková, R., Štěpánková, Prokop, Z., Kutá Smatanová, I., Hof, M., Damborský, J.: Dynamics and Hydration Explain Failed Functional Transformation in Dehalogenase Design, Nature Chemical Biology, 10, 428–430, 2014.
[BibTeX] [PDF]


sign in

E-mail:
Password:   

Create new user account

Forgot your password? Please contact us at caver@caver.cz.

user statistics

524 citations
5815 registered users
71325x CAVER downloaded

news

January 18, 2017

CAVER was cited in the NATURE journal in article Structure of a CLC chloride ion channel by...

Read more

January 18, 2017

CAVER was cited in the NATURE journal in article Crystal structure of the epithelial calcium channel...

Read more

January 18, 2017

CAVER was cited in the NATURE journal in article Structural basis of Smoothened regulation by its...

Read more


other tools

acknowledgement