A Single Mutation in the Access Tunnel Changes Mechanism of Product Release in Haloalkane Dehalogenase LinB

Authors

Biedermannová, L., Prokop, Z., Gora, A., Kovács, M., Damborský, J., Wade, R.C.

Source

. Journal of Biological Chemistry 287

Abstract

Many enzymes have buried active sites. The properties of the tunnels connecting the active site with bulk solvent affect ligand binding and unbinding and also the catalytic properties. Here, we investigate ligand passage in the haloalkane dehalogenase enzyme LinB and the effect of replacing leucine by a bulky tryptophan at a tunnel-lining position. Transient kinetic experiments show that the mutation significantly slows down the rate of product release. Moreover, the mechanism of bromide ion release is changed from a one-step process in the wild type enzyme to a two-step process in the mutant. The rate constant of bromide ion release corresponds to the overall steady-state turnover rate constant, suggesting that product release became the rate-limiting step of catalysis in the mutant. We explain the experimental findings by investigating the molecular details of the process computationally. Analysis of trajectories from molecular dynamics simulations with a tunnel detection software reveals differences in the tunnels available for ligand egress. Corresponding differences are seen in simulations of product egress using a specialized enhanced sampling technique. The differences in the free energy barriers for egress of a bromide ion obtained using potential of mean force calculations are in good agreement with the differences in rates obtained from the transient kinetic experiments. Interactions of the bromide ion with the introduced tryptophan are shown to affect the free energy barrier for its passage. The study demonstrates how the mechanism of an enzymatic catalytic cycle and reaction kinetics can be engineered by modification of protein tunnels.

Source

Biedermannová, L., Prokop, Z., Gora, A., Kovács, M., Damborský, J., Wade, R.C.: A Single Mutation in the Access Tunnel Changes Mechanism of Product Release in Haloalkane Dehalogenase LinB, . Journal of Biological Chemistry , 287, 29062-29074, 2012.
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