Crystallization and Crystallographic Analysis of the Rhodococcus rhodochrous NCIMB 13064 Mutant DhaA31 and its Complex with 1,2,3-Trichloropropane

Authors

Lahoda, M., Chaloupková, R., Stsiapanava, A., Damborský, J., Kutá Smatanová, I.

Source

Acta Crystallographica 67

Abstract

Haloalkane dehalogenases hydrolyze carbon-halogen bonds in a wide range of halogenated aliphatic compounds. The potential use of haloalkane dehalogenases in bioremediation applications has stimulated intensive investigation of these enzymes and their engineering. The mutant DhaA31 was constructed to degrade the anthropogenic compound 1,2,3-trichloropropane (TCP) using a new strategy. This strategy enhances activity towards TCP by decreasing the accessibility of the active site to water molecules, thereby promoting formation of the activated complex. The structure of DhaA31 will help in understanding the structure-function relationships involved in the improved dehalogenation of TCP. The mutant protein DhaA31 was crystallized by the sitting-drop vapour-diffusion technique and crystals of DhaA31 in complex with TCP were obtained using soaking experiments. Both crystals belonged to the triclinic space group P1. Diffraction data were collected to high resolution: to 1.31 Å for DhaA31 and to 1.26 Å for DhaA31 complexed with TCP.

Source

Lahoda, M., Chaloupková, R., Stsiapanava, A., Damborský, J., Kutá Smatanová, I.: Crystallization and Crystallographic Analysis of the Rhodococcus rhodochrous NCIMB 13064 Mutant DhaA31 and its Complex with 1,2,3-Trichloropropane, Acta Crystallographica , 67, 397-400, 2011.
[BibTeX] [PDF]


sign in

E-mail:
Password:   

Create new user account

Forgot your password? Please contact us at caver@caver.cz.

user statistics

524 citations
5848 registered users
75367x CAVER downloaded

news

August 7, 2017

CAVER was cited in the Proceedings of the National Academy of Sciences journal in article Structural...

Read more

July 11, 2017

CAVER was cited in the NATURE journal in article Electron cryo-microscopy structure of the...

Read more

January 18, 2017

CAVER was cited in the NATURE journal in article Structure of a CLC chloride ion channel by...

Read more


other tools

acknowledgement