Enantioselectivity of Haloalkane Dehalogenases and its Modulation by Surface Loop Engineering

Authors

Prokop, Z., Sato, Y., Brezovský, J., Mozga, T., Chaloupková, R., Koudeláková, T., Jeřábek, P., Štěpánková, V., Natsume, R., van Leeuwen, J.G.E., Janssen, D.B., Florián, J., Nagata, Y., Senda, T., Damborský, J.

Source

Angewandte Chemie 49

Abstract

Engineering of the surface loop in haloalkane dehalogenases affects their enantiodiscrimination behavior. The temperature dependence of the enantioselectivity (lnE versus 1/T) of β-bromoalkanes by haloalkane dehalogenases is reversed (red data points) by deletion of the surface loop; the selectivity switches back when an additional single-point mutation is made. This behavior is not observed for α-bromoesters.

Source

Prokop, Z., Sato, Y., Brezovský, J., Mozga, T., Chaloupková, R., Koudeláková, T., Jeřábek, P., Štěpánková, V., Natsume, R., van Leeuwen, J.G.E., Janssen, D.B., Florián, J., Nagata, Y., Senda, T., Damborský, J.: Enantioselectivity of Haloalkane Dehalogenases and its Modulation by Surface Loop Engineering, Angewandte Chemie , 49, 6111-6115, 2010.
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