tools
explore the features
of CAVER tools concept
learn more about
CAVER principles download
download latest build
and plugins help
user guide, examples,
publications

Engineering Protein Resistance to Organic Co-solvent and Elevated Temperature by Access Tunnel Modification

Authors

Koudeláková, T., Chaloupková, R., Brezovský, J., Prokop, Z., Pavlová, M., Hessler, M., Khabiri, M., Ettrich, R., Bornscheuer, U.T., Damborský, J.

Source

Angewandte Chemie International Edition 52

Abstract

Mutations targeting as few as four residues lining the access tunnel extended enzyme’s half-life in 40% dimethyl sulfoxide from minutes to weeks (4,000-fold) and increased its melting temperature by 19 °C. Protein crystallography and molecular dynamics revealed that the tunnel residue packing is a key determinant of protein stability and the active-site accessibility for co-solvent molecules (red dots). The broad applicability of this concept was verified by analyzing twenty six proteins with buried active sites from all six enzyme classes.

Source

Koudeláková, T., Chaloupková, R., Brezovský, J., Prokop, Z., Pavlová, M., Hessler, M., Khabiri, M., Ettrich, R., Bornscheuer, U.T., Damborský, J.: Engineering Protein Resistance to Organic Co-solvent and Elevated Temperature by Access Tunnel Modification, Angewandte Chemie International Edition , 52, 1959-1963, 2013.
[BibTeX] [PDF]

sign in

E-mail:
Password:

---

Create new user account

Forgot your password? Please contact us at caver@caver.cz.

HANDS-ON COMPUTATIONAL ENZYME DESIGN COURSE

user statistics

1219 citations
6648 registered users
112560x CAVER downloaded

news

other tools

acknowledgement