Enantioselectivity of Haloalkane Dehalogenases and its Modulation by Surface Loop Engineering

Authors

Prokop, Z., Sato, Y., Brezovský, J., Mozga, T., Chaloupková, R., Koudeláková, T., Jeřábek, P., Štěpánková, V., Natsume, R., van Leeuwen, J.G.E., Janssen, D.B., Florián, J., Nagata, Y., Senda, T., Damborský, J.

Source

Angewandte Chemie 49

Abstract

Engineering of the surface loop in haloalkane dehalogenases affects their enantiodiscrimination behavior. The temperature dependence of the enantioselectivity (lnE versus 1/T) of β-bromoalkanes by haloalkane dehalogenases is reversed (red data points) by deletion of the surface loop; the selectivity switches back when an additional single-point mutation is made. This behavior is not observed for α-bromoesters.

Source

Prokop, Z., Sato, Y., Brezovský, J., Mozga, T., Chaloupková, R., Koudeláková, T., Jeřábek, P., Štěpánková, V., Natsume, R., van Leeuwen, J.G.E., Janssen, D.B., Florián, J., Nagata, Y., Senda, T., Damborský, J.: Enantioselectivity of Haloalkane Dehalogenases and its Modulation by Surface Loop Engineering, Angewandte Chemie , 49, 6111-6115, 2010.
[BibTeX] [PDF]


sign in

E-mail:
Password:   

Create new user account

Forgot your password? Please contact us at caver@caver.cz.

HANDS-ON COMPUTATIONAL ENZYME DESIGN COURSE

user statistics

1219 citations
6405 registered users
112037x CAVER downloaded

news

CAVER was cited in NATURE Communications paper entitled Molecular mechanism underlying regulation of...

Read more

June 22, 2023

CAVER was recently cited by the NATURE publication entitled Structural basis of the binding of DNP...

Read more

May 14, 2023

CAVER was recently cited in Nature in paper In situ architecture of the ER–mitochondria encounter...

Read more


other tools

acknowledgement