Enantioselectivity of Haloalkane Dehalogenases and its Modulation by Surface Loop Engineering

Authors

Prokop, Z., Sato, Y., Brezovský, J., Mozga, T., Chaloupková, R., Koudeláková, T., Jeřábek, P., Štěpánková, V., Natsume, R., van Leeuwen, J.G.E., Janssen, D.B., Florián, J., Nagata, Y., Senda, T., Damborský, J.

Source

Angewandte Chemie 49

Abstract

Engineering of the surface loop in haloalkane dehalogenases affects their enantiodiscrimination behavior. The temperature dependence of the enantioselectivity (lnE versus 1/T) of β-bromoalkanes by haloalkane dehalogenases is reversed (red data points) by deletion of the surface loop; the selectivity switches back when an additional single-point mutation is made. This behavior is not observed for α-bromoesters.

Source

Prokop, Z., Sato, Y., Brezovský, J., Mozga, T., Chaloupková, R., Koudeláková, T., Jeřábek, P., Štěpánková, V., Natsume, R., van Leeuwen, J.G.E., Janssen, D.B., Florián, J., Nagata, Y., Senda, T., Damborský, J.: Enantioselectivity of Haloalkane Dehalogenases and its Modulation by Surface Loop Engineering, Angewandte Chemie , 49, 6111-6115, 2010.
[BibTeX] [PDF]


sign in

E-mail:
Password:   

Create new user account

Forgot your password? Please contact us at caver@caver.cz.

HANDS-ON COMPUTATIONAL ENZYME DESIGN COURSE

user statistics

1219 citations
6611 registered users
112455x CAVER downloaded

news

CAVER was recently cited in NATURE Communications in paper entitled Structural insights into drug...

Read more

April 15, 2024

CAVER was recently cited in NATURE paper entitled Bitter taste receptor activation by cholesterol...

Read more

CAVER was recently cited in the article entitled Constitute activation mechanism of a class C GPCR,...

Read more


other tools

acknowledgement