tools
explore the features
of CAVER tools concept
learn more about
CAVER principles download
download latest build
and plugins help
user guide, examples,
publications

Molecular Gating of an Engineered Enzyme Captured in Real Time

Authors

Kokkonen, P., Sykora, J., Prokop, Z., Ghose, A., Bednar, D., Amaro, M., Bidmanova, S., Beerens, K., Damborsky, J., Hof, M.

Source

Journal of the American Chemical Society 140

Abstract

Engineering dynamical molecular gates represents a widely applicable strategy for designing efficient biocatalysts. Here we analyzed the dynamics of a molecular gate artificially introduced into an access tunnel of the most efficient haloalkane dehalogenase using pre-steady-state kinetics, a single-molecule fluorescence spectroscopy and molecular dynamics. Photoinduced electron-transfer – fluorescence correlation spectroscopy (PET-FCS) has enabled real-time observation of molecular gating at single molecule level with the rate constants (kon = 1822 s-1, koff = 60 s-1) corresponding well with those from the pre-steady-state kinetics (k-1 = 1100 s-1, k1 = 20 s-1).

Source

Kokkonen, P., Sykora, J., Prokop, Z., Ghose, A., Bednar, D., Amaro, M., Bidmanova, S., Beerens, K., Damborsky, J., Hof, M.: Molecular Gating of an Engineered Enzyme Captured in Real Time, Journal of the American Chemical Society, 140, 17999–18008, 2018.
[BibTeX]

sign in

E-mail:
Password:

---

Create new user account

Forgot your password? Please contact us at caver@caver.cz.

HANDS-ON COMPUTATIONAL ENZYME DESIGN COURSE

user statistics

1219 citations
6405 registered users
112037x CAVER downloaded

news

other tools

acknowledgement