Molecular Gating of an Engineered Enzyme Captured in Real Time

Authors

Kokkonen, P., Sykora, J., Prokop, Z., Ghose, A., Bednar, D., Amaro, M., Bidmanova, S., Beerens, K., Damborsky, J., Hof, M.

Source

Journal of the American Chemical Society 140

Abstract

Engineering dynamical molecular gates represents a widely applicable strategy for designing efficient biocatalysts. Here we analyzed the dynamics of a molecular gate artificially introduced into an access tunnel of the most efficient haloalkane dehalogenase using pre-steady-state kinetics, a single-molecule fluorescence spectroscopy and molecular dynamics. Photoinduced electron-transfer – fluorescence correlation spectroscopy (PET-FCS) has enabled real-time observation of molecular gating at single molecule level with the rate constants (kon = 1822 s-1, koff = 60 s-1) corresponding well with those from the pre-steady-state kinetics (k-1 = 1100 s-1, k1 = 20 s-1).

Source

Kokkonen, P., Sykora, J., Prokop, Z., Ghose, A., Bednar, D., Amaro, M., Bidmanova, S., Beerens, K., Damborsky, J., Hof, M.: Molecular Gating of an Engineered Enzyme Captured in Real Time, Journal of the American Chemical Society, 140, 17999–18008, 2018.
[BibTeX]


sign in

E-mail:
Password:   

Create new user account

Forgot your password? Please contact us at caver@caver.cz.

user statistics

1219 citations
6071 registered users
111469x CAVER downloaded

news

CAVER was recently cited by the paper Structure of Escherichia coli cytochrome bd-II type oxidase...

Read more

CAVER was cited by the paper entitled Structural dynamics in the water and proton channels of...

Read more

November 2, 2021

CAVER was recently cited by the NATURE paper entitled Dispatched uses Na+flux to power release of...

Read more


other tools

acknowledgement